Dengue Infection and Transmission
Apply integrated glycomics approaches to understand and predict Dengue virus binding and infectivity to develop a platform for predictive and therapeutic application in severity of Dengue infection and other flavi-viruses in Singapore and region.
RNA-based Mechanisms in Dengue Pathogenesis
Development of a broad-based RNA platform for identification of therapeutic targets and fundamental understanding of viral pathophysiology and identification of antiviral drug targets.
Biomarkers of Dengue Infection
Discovery and application of biomarkers in the prognosis and intervention of Dengue infection with its impact on hospital management and healthcare cost in Singapore.
Humanized Mouse Model for Dengue Infection
Development of novel animal model platform of Dengue infection wth focus on viral pathophysiology and novel therapeutics.
Dengue vaccine formulation and transdermal microneedle delivery
Self-assembled thin film technologies and other encapsulations methods will be developed that can release RNA, DNA or proteins from the surfaces of microneedles, as well as an array of additional technologies directed toward transdermal delivery using microneedle platforms. This project is directed toward applying materials science and bioengineering approaches towards adapting these technologies in collaboration with the Dengue team to deliver mRNA, DNA or proteins for vaccines. Here we hope to expand the utility of this work to a new area of transdermal RNA and/or DNA vaccines against tropical disease.
Mechano-biology of Malaria Pathogenesis
Development of a uniquely comprehensive platform for discovery and elucidation of mechanics-based pathways in malaria pathogenesis offering an entirely new perspective on targets for developing malaria therapeutics.
RNA-based Mechanisms of Malaria Pathogenesis
Development of a broad-based platform for identification of RNA-based mechanisms controlling the malaria life cycle and the pathophysiology of infection, identifying new antibiotic targets and to improve the fundamental understanding of microbial pathophysiology and host-pathogen interactions.
Humanized Mouse Model of Malaria Infection
Development of novel animal model platform of malaria infection providing a unique tool to study the malarial pathophysiology and screening for novel therapeutics.
Bacterial Pathogens Program
Whole cell phenotypic screening tools for mycobacterial antibiotic development
The mycobacterial cell wall poses a problem for delivery of drugs to intracellular targets. To overcome this pharmacokinetic barrier, this project aims to develop new drug screening tools that take advantage of fluorescence-based phenotypic changes when the drug candidate both enters the cell and reacts with its target.
Antibiotic development based on translational control mechanisms in bacteria
This project exploits a recently discovered mechanism of translational control of bacterial survival responses to develop novel antibiotics based on rational drug design and high-throughput screening.
Bacterial PK/PD problems in drug resistance and drug discovery
This project addresses a new model for bacterial drug resistance and drug activation involving metabolism of drugs and prodrugs within the bacterial cell, including drug- and environmentally-induced up- and down-regulation of bacterial metabolic pathways.