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SMART researchers pave the way for safer T-cell therapy through contamination detection method

Dr James Strutt, Senior Postdoctoral Associate at SMART CAMP, operating the Oxford Nanopore GridION long read sequencer (Photo: SMART CAMP)

● Traditional sterility testing methods for the presence of bacteria and fungi in T-cell cultures are time-consuming, taking from seven up to 14 days, while this novel method takes only up to 24 hours

● Researchers combined advanced long-read nanopore sequencing techniques and machine learning to ensure accuracy and speed in detecting and identifying sample sterility status and microbial species present in T-cell cultures

● This breakthrough has the potential to transform sterility assurance in biopharmaceutical manufacturing, leading to better patient outcomes by accelerating the process of getting cell therapy treatments to patients

Singapore, 27 November 2023 - Researchers from Critical Analytics for Manufacturing Personalized-Medicine (CAMP) Interdisciplinary Research Group (IRG) at Singapore-MIT Alliance for Research and Technology (SMART), MIT’s research enterprise in Singapore, in collaboration with Singapore Centre for Environmental Life Sciences Engineering (SCELSE) and Massachusetts Institute of Technology (MIT), have developed a novel method capable of identifying contaminants in T-cell cultures within 24 hours. This method, adept at detecting low-abundance levels of microbial contaminants while effectively determining their types, ensures that therapeutic products like T-cell therapies are safe from contamination, with a faster turnaround – reducing the risk for patients and speeding up the process of getting these treatments to those in need.

Cell therapies are increasingly prevalent in the treatment of incurable diseases. For example, chimeric antigen receptor T-cells (CAR-T) are used for the treatment of blood-related cancers. Because these living medicines cannot be sterilised, assuring that cell therapy products are safe from microbial contamination before administering them for use in patients is of paramount importance.

Current traditional sterility tests help to guarantee microbial safety by ensuring that medical treatments are free from harmful contaminants. They improve the safety and efficiency of cell therapy manufacturing, playing an essential role in reducing the risk of treatments for patients. These traditional sterility methods are laborious and take between seven to 14 days; hence, faster methods could potentially be lifesaving for patients who cannot afford delays in their treatment. SMART CAMP’s breakthrough method could enhance both the safety and efficiency of cell therapy manufacturing, resulting in improved patient outcomes and a more streamlined production process.

In a paper titled “Machine learning-based detection of adventitious microbes in T-cell therapy cultures using long-read sequencing”, published recently in the journal Microbiology Spectrum, SMART researchers detailed a novel method to detect contaminants in T-cell cultures within 24 hours, significantly faster than traditional methods that can take 7-14 days. The researchers employed cutting-edge technology like third-generation nanopore long-read sequencing and DNA extraction alongside machine learning algorithms to improve the overall process and ensure accuracy and speed. The primary objective is to differentiate between clean and contaminated samples and pinpoint the organisms most likely causing the contamination.

SMART’s novel method utilises third-generation nanopore long-read sequencing methodology to identify harmful microorganisms, followed by an advanced machine learning algorithm, computational analysis and optimisation. Through this process, the researchers successfully identified the presence and types of microbial contaminants even at low abundance levels while also achieving this more quickly than standard compendial tests.

“The practical application of this discovery is vast; it offers faster product validation for biopharmaceutical manufacturers, reducing downtime and potentially accelerating product-to-market timelines. These advancements hold significant promise for the biopharmaceutical industry, as they not only enhance quality control but also improve overall efficiency and cost-effectiveness, ultimately benefiting patients by ensuring the safety and reliability of cell therapy products,” said Dr James Strutt, Senior Postdoctoral Associate at SMART CAMP and first author of the paper.

The research was presented at the A*STAR Mini Symposium in the Advances in Cell and Gene Therapy (A*CGT) in Singapore, and at the 31st Annual Intelligent Systems For Molecular Biology and the 22nd Annual European Conference on Computational Biology held in Lyon, France, by Dr James Strutt.

SMART CAMP is preparing to initiate testing to evaluate the integration of the novel T-cell sterility test into their processes and to further enhance the accuracy of contamination detection, while future research will focus on providing a similar level of detection for viruses and is currently being finalised at MIT. This advancement aims to provide more robust and dependable sterility assessments, in line with the SMART researchers’ core goal of swiftly and accurately identifying sample contamination.

“Our rapid method offers a more efficient way to not only detect microbial contamination, but identify the contaminating species. We demonstrated that this method can deliver a high-sensitivity microbial sterility assessment within just 24 hours, providing a valuable tool for researchers and hopefully practitioners in the near future,” added co-corresponding author Dr Stacy L. Springs, Principal Investigator at SMART CAMP and Executive Director at MIT Center for Biomedical Innovation.

The research is carried out by SMART and supported by the National Research Foundation (NRF) Singapore under its Campus for Research Excellence And Technological Enterprise (CREATE) programme.


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